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Figure 2 - Functional subclonal heterogeneity in DIPG. (a) Single cell-derived colonies were

established from the primary tumour DIPG006 and grown in the inner 60 wells of a laminin-coated 96

well plate under adherent stem cell culture conditions. Screen shot after 63 days, with representative

images of subclones C3 and B11 highlighting differential growth and morphology (b) Growth rates of

individual colonies monitored by the Celigo S. (c) Single cell-derived colonies grown in the inner 60

wells of round-bottomed 96 well plate under three dimensional neurosphere conditions. Screen shot

after 63 days, with representative images of subclones B10 and E3 highlighting differential growth of

spheroids. (d) Growth rates of individual neurospheres monitored by the Celigo S.

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